Claims 30
From the paper Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli · claims file · checked 2026-10-21: ready
- fig1c-app-approach
In a Y-maze preference test between saline and 2-phenylethanol, mice spend more time in the arm with the APP odor.
In a preference test between saline (SAL) and the APP odor, mice spent significantly more time in the arm with the APP odor ( t (16) = −5.7, p < 0.001; Figures 1B and 1C ), indicating approach to the APP odor.
Figure 1B and 1C; Results, APP and AV odors elicit innate behavioral responses, paragraph 1How it will be judged
Time in each arm from tracking over the 10 min session (Methods, Odor preference behavior tests), expressed as percent change of time in the odor arm against the saline arm (Figure 1C legend). What the t-test compares is open. Readings are (a) unpaired, SAL-vs-SAL mice against SAL-vs-APP mice on the percent change (the legends' groups; this gives df 15, not 16), (b) paired, time in the odor arm against the saline arm within the APP mice (df 8), and (c) unpaired, time in the odor arm against time in the saline arm across the 9 APP mice (Figure 1B shows time in each arm; df 16, as reported). Reproduced when p < 0.05 with more time near the APP odor under every reading; blocked as methods_underspecified when they disagree.
- fig1c-av-avoidance
In a preference test between saline and mountain lion urine, mice spend more time in the saline arm.
In contrast, in a preference test between SAL and the AV odor, mice spent significantly more time in the arm with SAL ( t (14) = −5.06, p < 0.001; Figures 1B and 1C ), indicating avoidance of the AV odor.
Figure 1B and 1C; Results, APP and AV odors elicit innate behavioral responses, paragraph 1How it will be judged
As in fig1c-app-approach, with readings (a) unpaired SAL-vs-SAL against SAL-vs-AV mice (df 14, as reported), (b) paired within AV mice (df 7) and (c) unpaired, odor arm against saline arm time across the 8 AV mice (df 14, as reported). Reproduced when p < 0.05 with more time in the saline arm under every reading; blocked as methods_underspecified when they disagree.
- figs1-locomotion-unchanged
Neither odor changes total distance traveled or velocity in the preference test, and there are no sex differences in approach or avoidance.
Neither the APP nor the AV odor affected total distance traveled or velocity ( Figures S1A and S1B ). We did not find any sex differences in approach or avoidance behavior in response to odor exposure ( Figures S1C and S1D ).
Results, APP and AV odors elicit innate behavioral responses, paragraph 1; Figure S1A to S1D (not in the text read)How it will be judged
A claim of absence. The test in Figure S1 is not in the text read; the Methods give a one-way ANOVA for three groups (SAL, APP, AV) and a two-tailed t-test for two (male against female), alpha 0.05. Reproduced when none of these finds a difference at p < 0.05; give the effect sizes.
- fig1f-cfos-total
The total number of cFos+ cells in the VP increases after exposure to either odor, while the number of ChAT+ neurons does not differ between groups.
The total number of cFos + cells in the VP significantly increased following exposure to either odor ( F (2, 64) = 12.79, p < 0.001; pairwise comparisons: APP vs. SAL [ t = 4.29, p < 0.001], AV vs. SAL [ t = 4.64, p < 0.001]; Figure 1F , right). ... The number of cholinergic neurons identified was equivalent between groups (ChAT counts; Figure 1F , left).
Figure 1F; Results, Approach and avoidance behaviors engage VP cholinergic neuronsHow it will be judged
Imaris counts in the whole VP (Methods, Quantification). The unit of analysis is open. The Methods average 4 to 6 slices per mouse, which gives df (2, 22); the reported df (2, 64) implies 67 observations, which fits neither 25 mice nor 4 to 6 slices per mouse (100 to 150). Readings are (a) per-mouse means and (b) per-slice counts. Reproduced when the ANOVA and both pairwise comparisons give p < 0.05 with more cFos+ cells after each odor. The equal ChAT counts are a claim of absence, judged by the same one-way ANOVA at alpha 0.05.
- fig1g-chat-cfos
Exposure to either odor increases the number of ChAT+ cFos+ VP neurons and the percentage of ChAT+ neurons that are cFos+ (about 20%).
Cholinergic neurons were activated following exposure to either odor (number of co-localized ChAT and cFos + neurons [ Figure 1G , left]: F (2, 64) = 4.19, p < 0.05; pairwise comparison: APP vs. SAL [ t = 2.77, p < 0.05], AV vs. SAL [ t = 2.26, p < 0.05]; percentage of ChAT neurons that were cFos + after odor exposure [ Figure 1G , right]: F (2, 64) = 4.35, p < 0.05; pairwise comparisons: APP vs. SAL [ t = 2.63, p < 0.05], AV vs. SAL [ t = 2.59, p < 0.05]).
Figure 1G; Results, Approach and avoidance behaviors engage VP cholinergic neuronsHow it will be judged
As in fig1f-cfos-total, with the same two readings of the unit. The 20% is stated in Results, Distinct and non-overlapping subpopulations, paragraph 1 ("equivalent numbers (~20%)"). Reproduced when all six tests give p < 0.05 with higher values after each odor and the percentage after each odor is within the tolerance.
- fig1k-app-calcium-auc
The AUC of the z-scored calcium signal of VP cholinergic neurons rises during APP odor deliveries compared with the pre-odor and post-odor periods.
The area under the curve (AUC) was significantly increased during each APP odor delivery ( F (4, 34) = 14.28, p < 0.001, Figure 1K ; pairwise comparisons: APP 1 vs. pre-odor [ t = 6.35, p < 0.001], APP 1 vs. post-odor [ t = 5.66, p < 0.001], APP 2 vs. pre-odor [ t = 3.02, p < 0.05], APP 3 vs. pre-odor [ t = 4.74, p < 0.001], APP 3 vs. post-odor [ t = 4.04, p < 0.05]).
Figure 1I and 1K; Results, VP cholinergic neurons display time-locked increases in calcium activity, paragraph 1How it will be judged
Signal from the 470 nm channel normalized to the 415 nm isosbestic channel (Methods; the code's normalization of the signal to its own biexponential fit is a variant), initialization frames (5 s) removed, 5 Hz bins, dF/F z-scored (Methods and code, whole session). AUC of the z-score in each 10 s delivery. The length of the pre-odor and post-odor windows is open, as is the design behind df (4, 34), which matches neither an independent (4, 30) nor a repeated measures (4, 24) ANOVA over 7 mice; readings are (a) 10 s windows before the first and after the last delivery and (b) the whole pre-odor and post-odor periods of the session. Reproduced when the ANOVA gives p < 0.05, each APP delivery exceeds pre-odor at p < 0.05 (as reported for all three) and APP 1 exceeds post-odor at p < 0.05.
- fig1l-av-calcium-auc
The AUC of the z-scored calcium signal rises during AV odor deliveries compared with the pre-odor and post-odor periods.
Likewise, the integrated Ca 2+ signal (AUC), was significantly increased following each AV odor delivery ( F (4, 34) = 12.34, p < 0.001, Figure 1L ; pairwise comparisons: AV 1 vs. pre-odor [ t = 5.78, p < 0.001], AV 1 vs. post-odor [ t = 5.15, p < 0.001], AV 2 vs. pre-odor [ t = 4.16, p < 0.05], AV 2 vs. post-odor [ t = 3.53, p < 0.05], AV 3 vs. pre-odor [ t = 3.81, p < 0.05], AV 3 vs. post-odor [ t = 3.19, p < 0.05]).
Figure 1J and 1L; Results, VP cholinergic neurons display time-locked increases in calcium activity, paragraph 1How it will be judged
As in fig1k-app-calcium-auc. Reproduced when the ANOVA gives p < 0.05 and each AV delivery exceeds pre-odor at p < 0.05.
- fig1mn-calcium-max-z
The maximum z-scored dF/F is higher during the first APP and the first AV odor delivery.
The maximum Z -score × F/F was significantly increased during the first APP odor ( F (4, 34) = 4.4, p < 0.05; t = 3.69, p < 0.05; Figure 1M ) and first AV odor ( F (4, 34) = 5.19, p < 0.05; t = 3.90, p <0.05; Figure 1N ).
Figure 1M and 1N; Results, VP cholinergic neurons display time-locked increases in calcium activity, paragraph 1How it will be judged
Max z-score dF/F is the highest z-score in the 10 s delivery (Methods); processing and the open windows as in fig1k-app-calcium-auc. The comparison the pairwise t names is not stated; readings are first delivery against pre-odor and against post-odor. Reproduced when each ANOVA gives p < 0.05 and the first delivery exceeds the comparison epoch at p < 0.05.
- fig2c-hm4di-app-reversal
Chemogenetic inhibition of VP cholinergic neurons (hM4Di) abolishes approach to the APP odor, and mice spend more time in the saline arm than in the APP arm.
In contrast, hM4Di inhibition of VP cholinergic neurons abolished this effect, and mice spent significantly more time in the SAL arm than in the arm with the APP odor ( t (8) = 4.21, p < 0.05; Figures 2B [right] and 2C ).
Figure 2B and 2C; Results, Silencing VP cholinergic neurons not only abolishes but reverses approach behavior, paragraph 1How it will be judged
What the t-test compares is open. Readings are (a) percent change of time in odor against saline arm (Figure 2C), sham (eGFP plus clozapine) against hM4Di mice, unpaired two-tailed t-test (df 8 matches 5 and 5), and (b) time in the saline arm against time in the APP arm across the 5 hM4Di mice, unpaired (the sentence's comparison; also df 8). Reproduced when p < 0.05 with the hM4Di mice spending more time in the saline arm under both readings; blocked as methods_underspecified when they disagree.
- fig2e-hm4di-av-unchanged
Chemogenetic inhibition of VP cholinergic neurons does not change avoidance of the AV odor, and does not change locomotion or time in each arm with saline in both arms.
Mice with hM4Di inhibition of VP cholinergic neurons also displayed avoidance of the AV odor ( Figures 2D [right] and 2E ). These results indicate that inhibition of cholinergic signaling in the VP was not sufficient to alter avoidance of the AV odor. These results were not due to any hM4Di-induced changes in behavior, as mice in both groups displayed comparable locomotor activity ( Figures S3A – S3D ). Furthermore, mice with hM4Di inhibition of VP cholinergic neurons displayed equal time spent in each arm during a preference test with SAL in both arms of the Y-maze ( Figures S3E and S3F ), ...
Figure 2D and 2E; Figure S3 (not in the text read); Results, Silencing VP cholinergic neurons, paragraph 2How it will be judged
A claim of absence, judged by an unpaired two-tailed t-test of sham against hM4Di mice (n = 7 and 7) at alpha 0.05 (Methods). Reproduced when it finds no difference and both groups avoid the AV odor (mean percent change below zero); give the effect size. The locomotion and SAL-SAL parts (Figure S3) have no test in the text read; they are judged by the same two-tailed t-test (sham against hM4Di for locomotion, saline arm against saline arm for the SAL-SAL test) and reported apart.
- fig3-same-odor-reactivation
When the same odor is given on both days, virtually all VP cholinergic neurons labeled by ADCD on day 1 are also cFos+ on day 2; saline on both days labels very few.
Notably, in each case, virtually all of the ADCD + VP cholinergic neurons (activated during the first exposure) were also cFos + (reactivated during the second exposure).
Figure 3B to 3D; Results, Distinct and non-overlapping subpopulations, paragraph 1How it will be judged
Counts of ChAT+ ADCD+, ChAT+ cFos+ and ChAT+ ADCD+ cFos+ neurons per mouse, averaged over 4 to 6 VP slices (Methods). No threshold for "virtually all" is given; reproduced when the reactivated count is at least 90% of the ChAT+ ADCD+ count in the APP/APP and AV/AV groups (n = 4 mice each) and SAL/SAL counts are far below both (the 90% is this file's reading of "virtually all", named as such).
- fig4d-different-odor-no-reactivation
Neurons activated by one odor are not reactivated by the opposite-valence odor a day later; the number of reactivated VP cholinergic neurons is far greater with the same odor on both days.
If mice were exposed to the same odor on days 1 and 2, there was a significantly greater number of reactivated VP cholinergic neurons ( F (3, 17) = 68.8, p < 0.001; Figure 4D , APP/APP and AV/AV compared to APP/AV and AV/APP), whereas if mice were exposed to a different odor on day 2, there was no overlap between ChAT + /ADCD + and ChAT + /cFos + VP cholinergic neurons.
Figure 4B to 4D; Results, Distinct and non-overlapping subpopulations, paragraphs 2 and 3How it will be judged
Reactivated counts (ChAT+ ADCD+ cFos+) per mouse across the four groups, one-way ANOVA (Methods). Reproduced when p < 0.001 and the APP/AV and AV/APP groups have zero, or essentially zero, reactivated neurons; partially reproduced when p < 0.05 with the same pattern.
- figs5-activation-counts
ChAT+ counts do not differ across the reactivation experiments, and both odors increase ADCD+ and cFos+ VP cholinergic neurons compared with saline, whichever day they are given.
Importantly, the number of ChAT + neurons was not significantly different across experiments ( Figure S5A ), and both odors increased the number of ADCD + ( Figure S5B ) and cFos + ( Figure S5C ) neurons, regardless of which day the odor was presented.
Figures S5A to S5C and S6 (not in the text read); Results, Distinct and non-overlapping subpopulations, paragraph 2How it will be judged
Tests in Figures S5 and S6 are not in the text read. Methods give one-way ANOVA across groups, alpha 0.05. Reproduced when ChAT+ counts show no difference and odor groups exceed SAL for ADCD+ and cFos+ at p < 0.05.
- figs7-ram-replication
The non-overlap of APP- and AV-activated VP cholinergic neurons is replicated with the RAM system in C57 mice.
We replicated these findings using the robust activity marking (RAM) system in C57 mice ( Figures S7 and S8 ).
Figures S7 and S8 (not in the text read); Results, Distinct and non-overlapping subpopulations, paragraph 3How it will be judged
No number or test for this replication is in the text read. Reproduced when the RAM data show reactivation with the same odor and essentially none with the opposite odor, as in fig4d-different-odor-no-reactivation.
- figs9-valence-specific
A second odor of the same valence reactivates most day-1 neurons, about 80% for two APP odors (2-phenylethanol then peanut butter oil) and about 70% for two AV odors (mountain lion urine then peppermint oil).
In these mice, ~80% of VP cholinergic neurons activated on day 1 were reactivated on day 2 ( Figures S9A and S9B ). ... In these mice, ~70% of day-1-activated VP cholinergic neurons were reactivated ( Figures S9C and S9D ).
Figure S9 (not in the text read); Results, Distinct and non-overlapping subpopulations, paragraph 3How it will be judged
Percentage is ChAT+ ADCD+ cFos+ over ChAT+ ADCD+. Whether it is pooled over mice or the mean of per-mouse percentages is open; both readings are run.
- fig5c-adcd-app-reversal
Selective inhibition (ADCD-hM4Di) of VP cholinergic neurons activated by the APP odor abolishes approach to it and produces avoidance.
However, selective silencing of VP cholinergic neurons that were previously activated by exposure to the APP odor abolished this behavior ( t (11) = 2.72, p < 0.05; Figures 5B [right] and 5C ). Particularly striking was that inhibition of previously activated APP odor VP cholinergic neurons not only blocked approach behavior but in fact elicited active avoidance of the APP odor.
Figure 5B and 5C; Results, Selective inhibition of VP cholinergic neurons previously activated by the APP odor, paragraph 1How it will be judged
Percent change of time in odor against saline arm on day 2 with clozapine, sham against ADCD-hM4Di, unpaired two-tailed t-test (df 11 matches 6 and 7). Reproduced when p < 0.05 with the inhibited group below zero.
- fig5e-adcd-av-unchanged
Selective inhibition of VP cholinergic neurons activated by the AV odor does not change avoidance of it, nor locomotion.
In sharp contrast to the dramatic effects of silencing APP-odor-activated VP cholinergic neurons, mice with ADCD-hM4Di silencing of VP cholinergic neurons previously activated by exposure to the AV odor had no effect ( Figures 5D [right] and 5E ). These results underscore our findings using hM4Di for non-selective inhibition of VP cholinergic neurons ( Figure 2 ) and were devoid of any changes in locomotor activity ( Figure S10 ).
Figure 5D and 5E; Figure S10 (not in the text read); Results, Selective inhibition, paragraph 2How it will be judged
A claim of absence, judged by an unpaired two-tailed t-test of sham (n = 5) against ADCD-hM4Di (n = 6) at alpha 0.05 (Methods). Reproduced when it finds no difference; give the effect size.
- figs11-intermingled
APP- and AV-odor-activated VP cholinergic neurons are spatially intermingled within the VP from bregma +0.62 to +0.14.
Across the targeted regions (bregma +0.62 to +0.14), we found that these differentially activated subgroups of VP cholinergic neurons are spatially intermingled within the VP ( Figure S11 ).
Figure S11 (not in the text read); Results, APP- vs. AV-odor-activated VP cholinergic neurons are intermingled, paragraph 1How it will be judged
Shown as a map with no test. Reproduced when cells of both groups occupy overlapping positions on each mapped section, with no section or VP subregion holding one group alone.
- fig6f-max-firing-rate
APP- and AV-odor-activated VP cholinergic neurons both have a higher maximum firing rate than home-cage VP cholinergic neurons.
Both APP and AV VP cholinergic neurons displayed a greater rate of maximum firing ( F (2, 38) = 10.71, pairwise comparisons: ALL vs. APP t (31) = 5.36, p < 0.01; ALL vs. AV t (27) = 2.80, p < 0.05; Figure 6F ) and sag potential ( Figure S12A ).
Figure 6F; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 2How it will be judged
Max firing rate as defined in the opening comment (Methods item 16; spike detection from the code v1.0.0). Cells are those of Figure 6 (APP 12, AV 8, ALL 21, compared with the dataset's counts); the Methods' exclusion rules (series resistance over 25 MOhm, input resistance under 50 MOhm, resting potential above -50 mV, or a change of more than 20% in any of these) are applied, and any rule the data do not allow to be checked is named. The test is open between two readings, both run; when they disagree the claim is blocked as methods_underspecified. (a) Cell-level one-way ANOVA over the three groups (df 2, 38) and uncorrected two-tailed unpaired t-tests for each pair (df 31 and 27, as reported). (b) The nested ANOVA the Methods name, cells nested in mice nested in group. Reproduced when both pairwise comparisons give p < 0.05 with APP and AV above ALL; the F is compared with 10.71 and the t values with 5.36 and 2.80, without deciding the outcome.
- figs12a-sag-higher
APP- and AV-odor-activated VP cholinergic neurons both have a larger sag potential than home-cage VP cholinergic neurons.
Both APP and AV VP cholinergic neurons displayed a greater rate of maximum firing ... and sag potential ( Figure S12A ).
Figure S12A (not in the text read); Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 2How it will be judged
Sag as defined in the opening comment (Methods item 2, with the code's smoothing and windows). The test in Figure S12A is not in the text read; the two readings of fig6f-max-firing-rate are used. Reproduced when APP and AV each exceed ALL at p < 0.05.
- fig6g-av-more-hyperpolarized
AV-odor-activated VP cholinergic neurons have a more hyperpolarized membrane potential than APP-odor-activated ones.
Specifically, AV-odor-activated VP cholinergic neurons were slightly more hyperpolarized than APP-odor-activated VP cholinergic neurons ( F (2, 38) = 11.39, p < 0.001; pairwise comparisons: APP vs. AV t (18) = 2.72, p < 0.05; Figures 6C and 6G ).
Figure 6C and 6G; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 3How it will be judged
Membrane potential with the two readings in the opening comment ((a) the code's baselineVm, (b) a segment with no injected current), crossed with the two test readings of fig6f-max-firing-rate. Reproduced when the ANOVA gives p < 0.05 and the APP against AV comparison gives p < 0.05 with AV more negative under every reading run; partially reproduced when only the pairwise comparison holds. If the readings disagree the claim is blocked as methods_underspecified, with every result shown.
- fig6h-av-smaller-ahp
AV-odor-activated VP cholinergic neurons have a smaller afterhyperpolarization amplitude than APP-odor-activated ones.
AV-odor-activated VP cholinergic neurons also had a smaller amplitude of the afterhyperpolarization potential ( F (2, 38) = 4.53, p < 0.05; pairwise comparisons: APP vs. AV, t = 2.74, p < 0.05; Figures 6D and 6H ).
Figure 6B (right), 6D and 6H; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 3How it will be judged
AHP amplitude per the Methods (item 12), with threshold at dV/dt 20 mV/ms, under the two readings in the opening comment ((a) the minimum within 100 ms after threshold, (b) the voltage at 100 ms); the code v1.0.0 definition is shown as a variant. Tests as the two readings of fig6f-max-firing-rate. Reproduced when the ANOVA and the APP against AV comparison give p < 0.05 with AV smaller under every Methods reading; blocked as methods_underspecified if they disagree.
- fig6-app-less-excitable
APP-odor-activated VP cholinergic neurons are less excitable than AV-odor-activated ones, with different action potential profiles.
The net effect of the differences in these features underlies the phenotype that APP VP cholinergic neurons are less excitable than AV VP cholinergic neurons.
Figure 6B to 6E; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 3How it will be judged
An interpretation with no test of its own. Figure 6B (middle) shows "differences in latency to fire an action potential" at rheobase. The measure of excitability is open; readings are (a) spike latency at rheobase (Methods item 9) longer in APP and (b) rheobase (item 5) higher in APP, each by the two test readings of fig6f-max-firing-rate at p < 0.05. Reproduced when every reading gives a significant difference in that direction, not reproduced when none does, blocked as methods_underspecified when they disagree.
- fig6j-convex-hull-area
The convex hull of APP-odor-activated VP cholinergic neurons has a smaller surface area (and volume) than that of AV-odor-activated neurons.
The convex hull enveloping APP-odor-activated VP cholinergic neurons was smaller in surface area ( F (1, 10) = 12.22, p < 0.05; Figure 6J ) and volume ( Figure S13C ) compared to AV-odor-activated VP cholinergic neurons.
Figure 6I and 6J; Figure S13C (not in the text read); Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 4How it will be judged
Convex hull from the Imaris filament reconstruction of the soma-proximal arbor (Methods, Neuronal morphology). Tests as the two readings of fig6f-max-firing-rate with two groups (df 1, 10 matches 12 cells). Reproduced when surface area is smaller in APP at p < 0.05; the volume has no test in the text read and is reported by the same test.
- fig6kl-dendrite-area-volume
APP-odor-activated VP cholinergic neurons have a smaller dendrite area and dendrite volume than AV-odor-activated neurons.
APP-odor-activated VP cholinergic neurons also exhibited a significantly smaller dendrite area ( F (1, 10) = 26.77, p < 0.05; Figure 6K ) and dendrite volume ( F (1, 10) = 57.31, p <0.05; Figure 6L ).
Figure 6K and 6L; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 4How it will be judged
Imaris filament dendrite area and volume. Tests as the two readings of fig6f-max-firing-rate with two groups. Reproduced when both are smaller in APP at p < 0.05.
- fig6m-sholl-proximal
APP-odor-activated VP cholinergic neurons have more Sholl intersections at 10 and 20 micrometres from the soma than AV-odor-activated neurons.
In addition to these features, APP-odor-activated VP cholinergic neurons had a significantly greater number of intersecting points in a Sholl analysis at distances close to the soma (at 10 μm F (1, 10) = −2.69, p <0.05; at 20 μm F (1, 10) = −3.16, p <0.05; Figure 6M ).
Figure 6M; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 4How it will be judged
Imaris Sholl intersections; the shell spacing is not given, and the two distances named (10 and 20 micrometres) imply a step of 10 micrometres at most. Tests as the two readings of fig6f-max-firing-rate with two groups at each distance. Reproduced when APP exceeds AV at both distances at p < 0.05.
- fig6n-sholl-reach
About 80% of AV-odor-activated VP cholinergic neurons have dendrites reaching at least 100 micrometres from the soma, against less than 20% of APP-odor-activated neurons.
The dendritic arbor of ~80% of AV-odor-activated VP cholinergic neurons extended at least 100 μm from the soma, whereas less than 20% of APP-odor-activated VP cholinergic neurons reached this distance ( Figure 6N ).
Figure 6N; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 4How it will be judged
Percentage of reconstructed cells (AV n = 6, APP n = 10, Figure 6M legend) with a Sholl intersection at 100 micrometres or beyond. No test is reported. Reproduced when AV is within 80 +- 8% and APP is below 22%. With 6 AV cells, 80% is not an exact fraction (5 of 6 is 83%).
- figs13-other-morphometrics
Other morphometric features do not differ between APP- and AV-odor-activated VP cholinergic neurons.
Additional morphometric features that were not statistically different between APP- and AV-odor-activated VP cholinergic neurons are shown in Figure S13 .
Figure S13 (not in the text read); Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 4How it will be judged
A claim of absence. Which features Figure S13 shows is not known here; the Methods' nested ANOVA at alpha 0.05 is the test. Testable only if the features are named, which needs the supplement.
- fig7d-bla-projecting-av
Equal numbers of VP cholinergic neurons are activated by the APP and AV odors, but a larger percentage of BLA-projecting (Fast Blue+) VP cholinergic neurons is activated by the AV odor.
Although we found an equal number of VP cholinergic neurons that were activated by the APP vs. AV odor ( Figure 7D , left), the relative proportion of BLA-projecting APP vs. AV VP cholinergic neurons was not equivalent. The percentage of BLA-projecting VP cholinergic neurons that were activated by the AV odor was significantly greater than the number of BLA-projecting VP cholinergic neurons activated by the APP odor ( t (6) = −2.98, p < 0.05; Figure 7D , right).
Figure 7D; Results, APP- vs. AV-odor-activated VP cholinergic neurons, paragraph 5How it will be judged
Counts per mouse averaged over slices (Methods). The percentage is open between (a) ChAT+ Fast Blue+ cFos+ over ChAT+ Fast Blue+ neurons in the VP (the sentence and the Figure 7D legend) and (b) ChAT+ Fast Blue+ cFos+ over ChAT+ cFos+ neurons (the "relative proportion of BLA-projecting APP vs. AV" neurons). Unpaired two-tailed t-test of AV against APP mice. Reproduced when p < 0.05 with AV higher under both readings, blocked as methods_underspecified when they disagree; the equal ChAT+ cFos+ counts (Figure 7D, left) are a claim of absence by the same test at alpha 0.05.
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