Claims 30
From the paper SRF transcriptionally regulates the oligodendrocyte cytoskeleton during CNS myelination · claims file · checked 2026-10-21: ready
- fig2c-cc-percent-myelinated
In the adult corpus callosum SRF-cKO mice have a lower percentage of myelinated axons than SRF-Flox littermates (about 22% against about 37%, read from the figure), so the hypomyelination seen in optic nerve persists into adulthood in corpus callosum.
This degree of hypomyelination of SRF-cKO mice persisted into adulthood in both the optic nerve ... and the corpus callosum
Figure 2C; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
Per mouse, the percent of axons myelinated, from the counts of myelinated and unmyelinated axon objects (SI Methods, Corpus callosum TEM; Figure 2C legend), under the readings O1, O2, O4 and O7 of the opening comment. Reproduced when, under every reading, the SRF-cKO mean is lower than the SRF-Flox mean with an unpaired two-tailed t test p < 0.05, and both group means are within tolerance. Partially reproduced when the direction and test hold but a group mean falls outside tolerance. The number of axons counted per mouse is reported against the paper's minimum of 2,500.
- fig2d-cc-myelinated-density
SRF-cKO adult corpus callosum has fewer myelinated axons per area than SRF-Flox (about 1.4 against about 1.8 per unit area, read from the figure).
Quantification of number of myelinated axons per area (at least 1,000 μm 2 ) in adult corpus callosum
Figure 2DHow it will be judged
Per mouse, the density of myelinated axons over the imaged area, under readings O1, O3, O4 and O7, with the image scale from the dataset's metadata. The imaged area per mouse is reported against the paper's minimum of 1,000 um2. Reproduced when, under every reading, the SRF-cKO mean is lower with an unpaired two-tailed t test p < 0.05 and the two means are within tolerance; partially reproduced when the direction and test hold and only the ratio is within tolerance; not reproduced otherwise.
- fig2e-cc-g-ratio
The average g-ratio of myelinated axons in adult corpus callosum is higher in SRF-cKO than in SRF-Flox mice (0.760 against 0.703).
the average g -ratio (ratio describing myelin thickness relative to axonal diameter) of SRF-cKO myelinated axons was significantly higher than WT littermates in the adult corpus callosum (0.760 vs. 0.703
Figure 2E; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
Per mouse, the mean g-ratio of its myelinated axons, under readings O1, O4, O5, O6 and O7. Reproduced when, under every reading, the SRF-cKO mean exceeds the SRF-Flox mean with an unpaired two-tailed t test p < 0.05 and both means are within tolerance. The 10% tolerance is wide for a g-ratio; the difference between the group means (0.057 in the paper) is reported beside it.
- figs3c-cc-myelin-thickness-unchanged
Average myelin thickness of myelinated axons in adult corpus callosum does not differ significantly between SRF-cKO and SRF-Flox mice (about 93 against about 105 nm, read from the figure), arguing against a role of SRF in myelin wrapping.
Interestingly, average myelin thickness was not significantly different between WT and SRF-cKO mice in either optic nerve or corpus callosum
Fig. S3C; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
A claim of absence, judged on the paper's test, an unpaired two-tailed Student t test (O7) on per-mouse mean myelin thickness at alpha 0.05, under readings O1, O4, O5 and O6. Reproduced when the test gives p >= 0.05 under every reading; not reproduced when it gives p < 0.05 under every reading; the group means and their difference are reported either way, with Welch's test as a variant.
- figs3d-cc-larger-myelinated-diameter
Myelinated axons in adult SRF-cKO corpus callosum have a larger mean diameter than in SRF-Flox (about 625 against about 530 nm, read from the figure), which accounts for the higher g-ratio and suggests a defect in ensheathing small-caliber axons.
Instead, the higher g -ratios observed in SRF-cKO mice were due to significantly larger diameters of myelinated axons in cKOs
Fig. S3D; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
Per mouse, the mean axon (inner) diameter of its myelinated axons, under readings O1, O4, O5, O6 and O7. Reproduced when, under every reading, the SRF-cKO mean exceeds the SRF-Flox mean with an unpaired two-tailed t test p < 0.05 and both means are within tolerance; partially reproduced when the direction and test hold but a mean is outside tolerance.
- figs2b-on-p18-half-myelinated
At P18 SRF-cKO optic nerves have about half as many myelinated axons as SRF-Flox littermates (about 16% against about 31% of axons, read from the figure), with no other abnormalities.
At P18 (during active myelination), SRF-cKO optic nerves had on average half as many myelinated axons as control SRF-Flox littermates with no other abnormalities
Fig. S2A and S2B; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
Not testable on this dandiset, which holds adult corpus callosum TEM only.
- figs2e-on-adult-hypomyelination
Hypomyelination persists in the adult optic nerve of SRF-cKO mice (about 49% against about 87% of axons myelinated, read from the figure).
This degree of hypomyelination of SRF-cKO mice persisted into adulthood in both the optic nerve
Fig. S2D and S2EHow it will be judged
Not testable on this dandiset, which holds adult corpus callosum TEM only.
- figs2cf-on-myelin-thickness-unchanged
Myelin thickness in optic nerve does not differ between SRF-cKO and SRF-Flox mice, at P18 or in adults.
average myelin thickness was not significantly different between WT and SRF-cKO mice in either optic nerve or corpus callosum
Fig. S2C and S2FHow it will be judged
Not testable on this dandiset, which holds adult corpus callosum TEM only.
- figs2ij-on-g-ratio-not-significant
In the optic nerve the higher g-ratio of SRF-cKO myelinated axons does not reach significance, at P18 or in adults.
but this result did not achieve significance in the optic nerve
Fig. S2I and S2J; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 2How it will be judged
Not testable on this dandiset, which holds adult corpus callosum TEM only.
- figs2kl-on-larger-myelinated-caliber
Myelinated axons in SRF-cKO optic nerve have a larger caliber than in SRF-Flox, at P18 and in adults, with the caliber distribution shifted to larger axons.
the higher g -ratios observed in SRF-cKO mice were due to significantly larger diameters of myelinated axons in cKOs
Fig. S2K to S2NHow it will be judged
Not testable on this dandiset, which holds adult corpus callosum TEM only.
- fig2gk-ol-lineage-density-unchanged
At P16 to P18, densities of OPCs (CC1-negative Olig2-positive) and oligodendrocytes (CC1-positive Olig2-positive) in corpus callosum and cortex do not differ between SRF-cKO and SRF-Flox mice, with a slight trend to fewer OPCs in cortex.
We found similar densities of OPCs and oligodendrocytes in both regions with a slight trend towards fewer OPCs in the cortex
Figure 2F to 2K; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 3How it will be judged
Not testable on this dandiset, which holds TEM images only, no immunostaining.
- figs3fg-mbp-intensity-unchanged
MBP staining intensity in corpus callosum and cortex at P16 to P18 is similar in SRF-cKO and SRF-Flox mice.
This was in line with similar levels of MBP staining intensity in the cortex and corpus callosum
Fig. S3E to S3G; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 3How it will be judged
Not testable on this dandiset, which holds TEM images only. The legends name an unpaired t test (n = 6 mice per genotype); no p-value is printed.
- fig1d-srf-expressed-opc-ol
Srf mRNA is expressed by both OPCs and oligodendrocytes throughout the brain at P8 and P16.
SRF mRNA was expressed by both OPCs and oligodendrocytes throughout the brain at both P8 and P16
Figure 1B to 1D; Results, SRF Is Expressed by OPCs and Oligodendrocytes during CNS Development, paragraph 1How it will be judged
Not testable on this dandiset, which holds no RNAscope data.
- fig1ef-srf-protein-nuclear
SRF protein is in oligodendrocyte nuclei in vivo, and nuclear in cultured OPCs and oligodendrocytes.
immunostaining with a knockout-validated antibody confirmed SRF protein expression in oligodendrocyte nuclei
Figure 1E and 1FHow it will be judged
Not testable on this dandiset, which holds no immunostaining.
- figs1-srf-loss-in-cko
SRF-cKO mice lose Srf mRNA in purified OPCs and SRF protein in oligodendrocytes but not in neighbouring cells such as neurons.
We confirmed complete loss of SRF mRNA in OPCs using RT-PCR on purified OPCs from SRF-cKO mice and littermate controls
Fig. S1B and S1C; Results, Conditional Knockout of SRF Causes CNS Hypomyelination, paragraph 1How it will be judged
Not testable on this dandiset, which holds no RT-PCR or immunostaining.
- fig3a-chip-peaks
SRF ChIP-seq in rat OPCs and day-3 oligodendrocytes finds 848 significant binding sites, narrowed to 329 high-confidence targets.
We identified 848 significant SRF binding sites (peaks) in total and narrowed down the list to 329 high-confidence targets
Figure 3A; Results, ChIP-Seq Identifies SRF Target Genes in Oligodendrocytes, paragraph 1How it will be judged
Not testable on this dandiset; the ChIP-seq data are in GEO.
- fig3bc-chip-tss-carg
Most SRF peaks lie within 2 kb of the transcription start site, and the peak sequences are enriched for the SRF CArG motif.
most peaks were enriched within 2 kb of the transcription start site (TSS) ... Inspection of the target sequences confirmed an enrichment for the SRF CArG consensus
Figure 3B and 3CHow it will be judged
Not testable on this dandiset; the ChIP-seq data are in GEO.
- fig4ab-rnaseq-degs
SRF knockout changes the expression of 157 genes in cultured OPCs and 264 in day-3 oligodendrocytes (adjusted p < 0.05).
We identified 157 differentially expressed genes ( P .adj < 0.05) in SRF-KO OPCs vs SRF-WT and 264 in SRF-KO oligodendrocytes
Figure 4A and 4B; Results, SRF Regulates Actin Cytoskeleton Gene Expression, paragraph 1How it will be judged
Not testable on this dandiset; the RNA-seq data are in GEO.
- fig4cd-rnaseq-srf-actb-down
Srf and Actb are down-regulated in SRF-KO OPCs and oligodendrocytes in culture, and actin genes among the CArG-containing direct targets are down-regulated.
Actin genes such as beta actin (Actb) were down-regulated in SRF-KO OPCs and oligodendrocytes
Figure 4C, 4D and 4H; Fig. S4A; Results, SRF Regulates Actin Cytoskeleton Gene Expression, paragraph 2How it will be judged
Not testable on this dandiset; the RNA-seq data are in GEO. The legends name DESeq2 statistics (Figure 4C and 4D) and a Wilcoxon rank-sum test (Figure 4H); the values printed on Figure 4 were not read.
- fig4eg-rnascope-in-vivo-down
In P16 to P18 cortex, Srf, Actb and Actg1 transcripts are reduced in Olig2-positive nuclei of SRF-cKO mice.
Srf ... Actb ... Actg1 ... were significantly down-regulated in our primary cells and in vivo in SRF-cKO Olig2 positive nuclei
Figure 4E to 4G; Fig. S4B and S4DHow it will be judged
Not testable on this dandiset, which holds no RNAscope data (n = 4 per genotype in the legends; the values printed on Figure 4 were not read).
- no-myelin-genes-direct-targets
Classical myelin genes are not among the direct SRF targets found by ChIP-seq.
Interestingly, we did not identify ... as direct SRF targets
Results, SRF Regulates Actin Cytoskeleton Gene Expression, paragraph 2How it will be judged
Not testable on this dandiset; the ChIP-seq data are in GEO.
- figs4ef-assembly-not-disassembly-genes
In cultured OPCs and oligodendrocytes, SRF mainly regulates genes that promote actin assembly, actin itself included, and not the main actin disassembly genes such as Cfl1.
SRF primarily regulates genes that promote actin assembly (including actin itself) and not genes that regulate actin disassembly
Fig. S4E and S4F; Results, SRF Functionally Regulates the Oligodendrocyte Actin Cytoskeleton, paragraph 1How it will be judged
Not testable on this dandiset; the RNA-seq data are in GEO. The Fig. S4F legend names DESeq2 statistics; no value is printed in the text.
- fig5e-less-f-actin
Cultured SRF-cKO OPCs and early-differentiating oligodendrocytes have much lower actin filament (phalloidin) levels, about 20% to 40% of SRF-Flox levels.
In OPCs and early stages of oligodendrocyte differentiation, SRF-cKO cells had dramatically reduced actin filament levels (~20% to 40% of SRF-Flox levels
Figure 5B, 5C and 5E; Results, SRF Functionally Regulates the Oligodendrocyte Actin Cytoskeleton, paragraph 1How it will be judged
Not testable on this dandiset, which holds no culture imaging.
- fig5d-late-actin-similar
Both genotypes disassemble actin during late differentiation, and by 7 days the two are nearly indistinguishable, though Figure 5E still marks the day-7 difference with two asterisks.
both SRF-Flox and SRF-cKO oligodendrocytes underwent a similar degree of actin disassembly during late stages of differentiation, and by full maturation at 7 d of differentiation cells from both genotypes were nearly indistinguishable
Figure 5D and 5E; Results, SRF Functionally Regulates the Oligodendrocyte Actin Cytoskeleton, paragraph 1How it will be judged
Not testable on this dandiset, which holds no culture imaging.
- culture-mbp-unchanged
MBP protein level does not differ between cultured SRF-WT and SRF-cKO oligodendrocytes.
the protein level of the differentiation marker and major myelin protein MBP was not different between SRF-WT and SRF-cKO oligodendrocytes
Results, SRF Functionally Regulates the Oligodendrocyte Actin Cytoskeleton, paragraph 1How it will be judged
Not testable on this dandiset, which holds no culture imaging. No figure or test is given for this statement.
- figs5ab-gsea-bulk
In bulk RNA-seq, SRF-KO OPCs are depleted of transcription regulation and cell cycle pathways, SRF-KO oligodendrocytes of actin cytoskeleton, postsynaptic density and myelin sheath pathways, and SRF-KO OPCs are enriched for disease pathways carrying genes such as Apoe and Clu.
enriched pathways in SRF-KO OPC genes also consisted of genes like Apoe and Clusterin (Clu) associated with diseases
Fig. S5A and S5B; Results, SRF Loss Induces Oligodendrocyte Disease-Associated Genes, paragraph 1How it will be judged
Not testable on this dandiset; the RNA-seq data are in GEO.
- fig6b-snrnaseq-degs
In 10-month-old prefrontal white matter, single-nucleus RNA-seq of SRF-cKO oligodendrocyte clusters finds 63 up-regulated and 213 down-regulated genes.
identified 63 significantly up-regulated and 213 down-regulated genes on both OL clusters combined
Figure 6A and 6B; Results, SRF Loss Induces Oligodendrocyte Disease-Associated Genes, paragraph 2How it will be judged
Not testable on this dandiset; the snRNA-seq data are in GEO.
- fig6cd-ol-genes-depleted
In SRF-cKO oligodendrocytes, oligodendrocyte-specific, actin cytoskeleton and mRNA processing pathways are depleted, endothelial and immune pathways enriched, and oligodendrocyte marker and myelin genes are among the top down-regulated genes.
Oligodendrocyte marker genes and myelin genes were among the top down-regulated genes
Figure 6C and 6DHow it will be judged
Not testable on this dandiset; the snRNA-seq data are in GEO.
- fig6eg-disease-genes-up
C4b, Serpina3n and Piezo2 are among the top up-regulated genes in SRF-cKO oligodendrocytes, and are absent or very low in 10-month-old SRF-Flox mice.
among the top upregulated genes in SRF-cKO oligodendrocytes were genes like C4b ... and Serpina3n ... Notably, these genes were absent or very lowly expressed in middle-aged (10-mo-old) SRF-Flox mice
Figure 6E to 6G; Results, SRF Loss Induces Oligodendrocyte Disease-Associated Genes, paragraph 3How it will be judged
Not testable on this dandiset; the snRNA-seq data are in GEO.
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