Claims 27
From the paper Post-ejaculatory inhibition of female sexual drive via heterogeneous neuronal ensembles in the medial preoptic area · claims file · checked 2026-10-22: ready
- fig2-density-link
The tdTomato+ cell densities computed from the dandiset's whole-brain images agree, brain by brain, with the densities in the paper's deposited table (tdTomato_summary.csv on Figshare) that its Figure 2 code reads.
Whole brain FosTRAP activity mapping data is available on DANDI
Data availability; Figure 2.ipynb at GitHub commit 4e98c43, cell 4How it will be judged
Densities computed here as in fig2c-brain-wide-increase (ClearMap registration to the Unified Anatomical Atlas, ilastik segmentation, count per region over region volume). The trained classifier is not deposited, so it is retrained as the Methods describe (10 random chunks of 1.8 x 1.8 mm x 100 um from four subjects). The link holds (reproduced) when the 14 brains fall into groups of 4, 5 and 5 matching the table's Stim labels and, for each brain, the median over level-6 regions of the absolute relative difference from the table's density of the matched subject is within 10% (the protocol's default tolerance). Partially reproduced when the groups match but fewer than 10 of the 14 brains meet the 10% bound. The per-brain correlation over regions is reported. The table is outside data (Figshare doi:10.6084/m9.figshare.27642768.v1).
- fig2c-brain-wide-increase
TRAP-labeled (tdTomato+) cell density increases brain-wide when 4-OHT is given after male ejaculation, compared with the appetitive phase.
As a result, we found the level of tdTomato expression increased in a brain-wide manner during the post-ejaculatory period when compared to the appetitive phase
Results, Brain-wide mapping of neural response to male ejaculation, paragraph 1; Figure 2C and 2DHow it will be judged
Method from the Methods (Brain-wide analysis) and the Figure 2 legend, with the code where they are silent. Per brain, tdTomato+ cells per atlas region (Unified Anatomical Atlas, Kim lab, outside data; Figure 2.ipynb reads its label table atlas_info_KimRef_FPbasedLabel_v2.9) divided by region volume (cells per mm3), at hierarchy level 4. Per region a one-way ANOVA over the three groups (appetitive n=4, consummatory n=5, post-ejaculatory n=5), Benjamini-Hochberg correction at FDR 0.05 (Methods and legend; the code uses 0.1, shown as a variant), then Tukey's HSD (FWER 0.05) post-ejaculatory against appetitive for regions significant in the ANOVA. Open, and named here because it could change the outcome; (1) the level-4 family is the ten regions of the code or the twelve of the Figure 2C legend (adding VERM and HEM); (2) region volume is the atlas volume or the region's volume in each brain's own space; the paper gives neither. Supplementary file 2, which lists which regions were significant, is not in the text read, so "brain-wide" is read as follows, stated before analysis. Reproduced when the post-ejaculatory mean exceeds the appetitive mean in at least 80% of the level-4 regions of the family and at least half of the family is significant (ANOVA q < 0.05 and Tukey p < 0.05, post-ejaculatory higher). Partially reproduced when the direction holds in at least 80% but fewer than half are significant. Not reproduced otherwise. Every reading is run; when they give different outcomes the claim is blocked as methods_underspecified.
- fig2ei-social-regions-higher
Regions known for social behavior, including the paraventricular hypothalamic areas (PVHd, PVH, PaV), the medial amygdala and the BNST, have significantly higher tdTomato+ density in the post-ejaculatory group than in the appetitive group.
regions well known to be related to social behavior had significantly higher tdTomato density in the post-ejaculatory group compared to appetitive group ... This includes paraventricular hypothalamic areas (PVHd, PVH, PaV) which are important for endocrinological regulations, medial amygdala (MeA) which is critical for olfactory sensory information processing, and the BNST
Results, Brain-wide mapping of neural response to male ejaculation, paragraph 1; Figure 2E to 2H; Figure 2-figure supplement 2How it will be judged
Densities as in fig2c-brain-wide-increase, at hierarchy level 6. Per region a one-way ANOVA over the three groups with Benjamini-Hochberg correction at FDR 0.10 over the level-6 family (Methods; Figure 2-figure supplement 2 legend), then Tukey's HSD at FWER 0.05 (Methods; p-values from scipy tukey_hsd as in Figure 2.ipynb). A region is significantly higher when q < 0.10, Tukey p < 0.05 and the post-ejaculatory mean is higher. The medial amygdala is the atlas label Me (Figure 2H legend); the other names are matched to atlas acronyms at level 6, or at the nearest level that holds them when level 6 does not (open; named before analysis). The level-6 family is (a) the code's, regions under the ten level-4 regions, or (b) all level-6 regions. Reproduced when all five regions (PVHd, PVH, PaV, Me, BNST) are significantly higher under the readings; partially reproduced for three or four; not reproduced below three. Per-region q and Tukey p are reported.
- fig2-mpn-higher
The medial preoptic nucleus (MPN) has significantly higher tdTomato+ density in the post-ejaculatory group than in the appetitive group.
The medial preoptic area nucleus (MPN), a subregion of the MPOA, had significantly higher tdTomato density in the post-ejaculatory group compared to the appetitive group
Results, Brain-wide mapping of neural response to male ejaculation, paragraph 1; Figure 2F and 2HHow it will be judged
As in fig2ei-social-regions-higher, for the atlas region MPN. Reproduced when the level-6 ANOVA q < 0.10 (under both family readings) and Tukey's HSD post-ejaculatory against appetitive p < 0.05 with the post-ejaculatory mean higher. Partially reproduced when Tukey p < 0.05 but q >= 0.10. The density ratio of the two group means is reported.
- fig2f-mpn-fourth-largest
The MPN shows the fourth largest difference in mean tdTomato+ density between the post-ejaculatory and appetitive groups among all brain regions.
The MPN also had the fourth largest difference of density between the post-ejaculatory group and appetitive group among all the brain regions
Results, Brain-wide mapping of neural response to male ejaculation, paragraph 1; Figure 2FHow it will be judged
Difference is the post-ejaculatory group mean minus the appetitive group mean of density (Figure 2F, "difference of average tdTomato density"). The set ranked is open; the readings the text allows are (a) the level-6 family of the code, (b) all level-6 regions, (c) all atlas regions at every level. The rank is a reported count (tolerance 0); the claim is reproduced when the MPN ranks fourth under one reading. The ranks under every reading and the three regions above the MPN are shown.
- fig2gh-consummatory-tendency
The consummatory group tends to have higher MPN tdTomato+ density than the appetitive group, without reaching significance.
While it was not statistically significant, the consummatory group also tended to have a larger tdTomato density than the appetitive group in the MPN as well
Results, Brain-wide mapping of neural response to male ejaculation, paragraph 1; Figure 2G and 2HHow it will be judged
For the MPN, Tukey's HSD consummatory against appetitive (FWER 0.05), as in Figure 2G and Figure 2.ipynb. The absence part is judged on that test alone; the claim is reproduced when Tukey p >= 0.05 and the consummatory mean is above the appetitive mean; partially reproduced when p >= 0.05 and the mean is not higher; not reproduced when p < 0.05. The mean difference and p are reported.
- fig2s-dorsal-raphe
The whole-brain mapping indicates increased TRAP labeling in the dorsal raphe after male ejaculation.
Our whole brain activity mapping data also indicates increased Fos activity in the dorsal raphe
Discussion, Neural circuitry for female sexual behavior regulation, last paragraph; Figure 2-figure supplement 1 and 2How it will be judged
For the atlas dorsal raphe region at level 6 (DR, or the label the atlas gives it), as in fig2ei-social-regions-higher. Reproduced when the post-ejaculatory mean exceeds the appetitive mean and the ANOVA q < 0.10 (the significance marked in Figure 2-figure supplement 2); partially reproduced when the mean is higher but q >= 0.10.
- fig1gh-transitions-isolation
In hormone-primed females, zone transitions decrease and time in the isolation zone increases after male ejaculation, compared with before ejaculation and with the control trial.
we found that there was a significant decrease in the number of zone transitions as well as a significant increase in the time spent in the isolation zone after male ejaculation
Figure 1G and 1H; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
As in the Figure 1 legend and Figure 1.ipynb; repeated-measures ANOVA (statsmodels AnovaRM) over control, pre- and post-ejaculation, then Tukey's HSD (FWER 0.05). Reproduced when both ANOVAs have p < 0.05 and post differs from pre and from control in the stated direction at p < 0.05.
- fig1i-latency-return
Latency to return to the interaction zone is longest after male ejaculation, longer than after sniffing or mounting.
This was the largest after the female experienced male ejaculation when compared to after sniffing or after mounting
Figure 1I; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
RM ANOVA then Tukey's HSD as in the Figure 1 legend. Reproduced when ejaculation latency exceeds both others at p < 0.05.
- fig1jm-behaviors-after-ejaculation
After ejaculation, female-to-male sniffing does not change, while mounting and intromission decrease and self-grooming increases (hormone-primed females).
while there was no change in the amount of female-to-male sniffing, there was a significant decrease in the number of mounting and intromission episodes ... There was also a significant increase in the number of self-grooming behavioral episodes
Figure 1J to 1M; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
Two-sided Wilcoxon signed-rank test pre against post on counts per 10 min (legend; Figure 1.ipynb, scipy wilcoxon). Reproduced when mounting and intromission decrease and grooming increases at p < 0.05, and sniffing is not significant (the absence judged on this test).
- fig1nt-natural-estrus
Naturally cycling estrus females show the same post-ejaculatory changes, with a strong reduction of sniffing and no change in self-grooming.
Animals that were in their natural estrus cycle showed similar behavior changes after experiencing male ejaculation ... showed a strong reduction of sniffing after experiencing male ejaculation but no changes in self-grooming behaviors
Figure 1N to 1T; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
Tests as in the Figure 1 legend (RM ANOVA and Tukey's HSD for N to P; Wilcoxon signed-rank for Q to T). Reproduced when the three ANOVAs, sniffing, mounting and intromission are significant at p < 0.05 (counts lower after ejaculation) and grooming is not (the absence judged on the Wilcoxon signed-rank test).
- fig1s1-experience
Virgin females spend more time in the isolation zone than sexually experienced females; the number of isolation zone entries also differs between the two groups.
the virgin female mice stayed in the isolation zone significantly longer than sexually experienced females
Figure 1-figure supplement 1; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
Two-sided Mann-Whitney U test (legend; Figure S1.ipynb, scipy mannwhitneyu). The legend does not say which part of the session is compared; Figure S1.ipynb (cell 8) compares the pre-ejaculation epoch of the sexual interaction trial, which is followed. Reproduced when the time difference is significant at p < 0.05 with virgin females higher and the entries difference is significant at p < 0.05. The text does not state the direction of the entries difference, which is reported.
- fig1s2-novel-male
The post-ejaculatory reduction in sexual motivation does not depend on the state of the male; with a novel sexually active male after ejaculation, females still make fewer isolation zone entries and spend more time there.
When the female animal was introduced to a novel sexually motivated male animal immediately after they experienced male ejaculation from another mouse, they showed a similar reduction in zone transition and an increase in the time spent in the isolation zone
Figure 1-figure supplement 2; Results, Female sexual motivation is suppressed after male ejaculation, paragraph 2How it will be judged
Two-sided Wilcoxon signed-rank test, pre- against post-ejaculation assay (legend; Figure S2.ipynb). Reproduced when both are significant at p < 0.05 in the stated direction.
- fig3d-mpn-fos
By HCR, the proportion of Fos+ cells is higher after ejaculation than in the appetitive group in the MPN but not in nearby regions (MPA, BNST, LPO, VLPO).
we found that the number of Fos + cells in the post-ejaculatory group was significantly higher than the appetitive group in the MPN but not in the nearby regions
Figure 3D; Results, Male ejaculation is represented by both inhibitory and excitatory cell types in the MPOA, paragraph 2How it will be judged
Per mouse and region, the proportion of Fos+ cells averaged over sections (Figure S5.ipynb groupby mean). Student's t-test with Bonferroni correction over the five regions (legend; Figure S5.ipynb, scipy ttest_ind and multipletests). Reproduced when only the MPN is significant at corrected p < 0.05, higher after ejaculation.
- fig3i-vgat-vglut2-fos
In the MPN, both Vglut2+ and Vgat+ cells expressing Fos increase after ejaculation, with a higher proportion of Fos+ cells in the Vgat population.
Vglut2 and Vgat cells expressing Fos were significantly higher in the post-ejaculatory group than in the appetitive group, suggesting that male ejaculation activates both excitatory and inhibitory neurons ... The proportion of Fos+ cells was higher in the Vgat population
Figure 3I; Results, Male ejaculation is represented by both inhibitory and excitatory cell types in the MPOA, paragraph 2How it will be judged
As in the Figure 3 legend. Reproduced when both are significant at corrected p < 0.05 and the post-ejaculatory Fos+ proportion is higher in Vgat than in Vglut2 cells (no test is reported for the latter, so it is judged on the means).
- fig3jm-cell-types
Fos induction after ejaculation is found in Vglut2 cells only when they co-express Calcr, and in Vgat cells co-expressing Calcr, Gal or Prlr; among clusters, the Vglut2, Vgat and Gal types have more Fos+ cells after ejaculation, while the Nts type does not.
in the Vglut2 cells, we found that Fos induction was only observed in the cells that co-expressed Calcr ... Type 1 ... and type 3 ... had significantly more Fos+ cells from the post-ejaculatory group compared to the appetitive group ... In contrast, Fos+ cells were not induced in type 4
Figure 3J to 3M; Results, Male ejaculation is represented by both inhibitory and excitatory cell types in the MPOA, paragraph 2How it will be judged
Student's t-tests with Bonferroni correction as in the Figure 3 legend; clusters from Seurat 3.1.1 as in the Methods and Figure S5_Seurat clustering.R. The legend prints t(8)=-3.9487 for every Vglut2 row and for Vgat+ Prlr+, so those statistics are not recorded. Reproduced when the named groups are significant at corrected p < 0.05 and the Nts cluster is not.
- fig4h-response-magnitude
Vgat cells show higher average response magnitude than Vglut2 cells after sniffing, self-grooming, male anogenital sniffing and ejaculation, but not after mounting or intromission.
Vgat cells showed significantly higher average response magnitude after sniffing, self-grooming, anogenital sniffing, and male ejaculation than the Vglut2 cells
Figure 4H; Results, A subpopulation of MPOA Vgat cells responds to male ejaculation, paragraph 1How it will be judged
Per cell, z-scored signal (z-scored on the background period) from -5 to +15 s around each onset, averaged over events, baseline (-5 to 0 s) subtracted, magnitude the mean of 0 to +5 s (Methods; the code's parameter_dict also sets after_window_for_quantification to 5 s). The Results say "for 15 s after behavior onset"; the Methods' 0 to 5 s is followed. Sniffs after the first mount are excluded (Methods). Student's t-test per behavior over the six behaviors with Bonferroni correction (legend; Figure 3, 4, 5, S6, S7, S8.ipynb, scipy ttest_ind and multipletests). Grooming has t = -7.25, that is Vgat lower, against the Results sentence; the sign is reported and grooming is left out of the outcome. Reproduced when ejaculation, sniff and anogenital sniffing are significant with Vgat higher and mount and intromission are not.
- fig4i-ejaculation-responders
A larger proportion of Vgat than Vglut2 cells respond positively to ejaculation (184/522 against 35/271) and to sniffing (29/522 against 1/271).
Vgat cells also contained a significantly higher proportion of cells that positively respond (>2δ) to sniff and male ejaculation onset
Figure 4I; Results, A subpopulation of MPOA Vgat cells responds to male ejaculation, paragraph 1How it will be judged
Responding when the magnitude of fig4h-response-magnitude exceeds 2 (Methods; abs value above 2 in the code, sign giving positive or negative). Chi-square test of proportions (statsmodels proportions_chisquare) over the six behaviors with Bonferroni correction (legend and Figure 3, 4, 5, S6, S7, S8.ipynb). Reproduced when the counts match and ejaculation and sniff differ at corrected p < 0.05 with Vgat higher.
- fig5-ejaculation-specific
Among cells responding to sniff, mount or ejaculation, ejaculation-specific cells are the most numerous (177, against 46 mount and 12 sniff), and most ejaculation responders are specific (Vgat 80%, Vglut2 82%).
the number of cells that specifically responded was largest to male ejaculation with 177 cells, next to mount with 46 cells, and to sniff with 12 cells
Results, A subpopulation of MPOA Vgat cells responds to male ejaculation, paragraph 2; Figure 5A to 5DHow it will be judged
The Figure 5 legend gives n=48 mount and 178 ejaculation cells, against 46 and 177 in the Results; both are readings. "Specific" is read as (a) positive (>2) to that behavior only, among sniff, mount and ejaculation, recomputed from the per-behavior positive responder flags of fig4i-ejaculation-responders, or (b) the precomputed magnitude_cell_types column of male_analysis_magnitude.csv, whose values Sniff_only_cells, Mount_only_cells and Ejaculation_only_cells the code uses for Figure 5 (the legend says the cells "were categorized based on response magnitude"). The counts hold if they match under one reading.
- fig5c-class-selectivity
Sniff-specific and ejaculation-specific cells respond more strongly than the other classes only to sniff and to ejaculation, respectively; mount-specific cells respond more strongly than the other classes to mount, intromission and anogenital sniffing, but not to sniff or ejaculation.
sniff cells and completion cells only showed significantly stronger responses than the other cells to sniff and male ejaculation, respectively ... Mount responsive cells showed significantly stronger response than the other cells to mount, intromission and anogenital sniffing, but not to sniffing and male ejaculation
Results, A subpopulation of MPOA Vgat cells responds to male ejaculation, paragraph 2; Figure 5CHow it will be judged
Cells are the sniff, mount and ejaculation-specific classes of fig5-ejaculation-specific; the measure is the response magnitude of fig4h-response-magnitude for each of the six behaviors. Per behavior a one-way ANOVA over the three classes and Tukey's HSD (FWER 0.05), as the panel C entries of the Figure 5 legend give them and as Figure 3, 4, 5, S6, S7, S8.ipynb runs them (statsmodels ols with anova_lm, pairwise_tukeyhsd). The legend's closing line names Student's t-test with Bonferroni correction for C; the reported F and Tukey values and the code both use ANOVA and Tukey, which is followed. Reproduced when, at p < 0.05, sniff cells exceed both other classes for sniff, ejaculation cells exceed both for ejaculation, mount cells exceed both for mount and exceed ejaculation cells for intromission and anogenital sniffing, and mount cells do not exceed sniff cells for sniff nor ejaculation cells for ejaculation.
- fig6c-decay-length
Ejaculation-responding Vgat cells have a longer decay length than Vglut2 cells.
Vgat cells had significantly longer decay length compared to the Vglut2 cells
Figure 6C; Results, MPOA Vgat cells display prolonged activity, paragraph 1How it will be judged
Signal from -5 to +120 s around ejaculation onset, baseline (-5 to 0 s) subtracted (Methods). Decay length is "the time the z-scored signal was smaller than 0" (Methods) or "the time for the increased calcium signal to return to baseline" (Results); the code reads it precomputed. Readings, (a) time from onset to the first sample below 0, (b) total time below 0 in 0 to 120 s. Student's t-test (scipy ttest_ind). Reproduced when Vgat is longer at p < 0.05 under the readings.
- fig6k-late-cluster-vgat
Spectral clustering of ejaculation responses gives five types; the late-response type 3 has the longest decay length and contains only Vgat cells, while Vglut2 cells are more often fast responders.
all the cells in type 3 were Vgat cells, suggesting that the increased activity in the MPOA late after the onset of male ejaculation is mediated by inhibitory neurons
Figure 6D to 6K; Results, MPOA Vgat cells display prolonged activity, paragraph 1How it will be judged
Cells are the ejaculation responders of fig4i-ejaculation-responders (184 Vgat, 35 Vglut2). PCA of the -5 to +120 s traces (whitened in Figure 5 Spectral clustering.ipynb), 4 components (Methods; the code picks the number at the elbow of the scree plot), spectral clustering (scikit-learn, nearest_neighbors affinity) with the number of clusters (2 to 11 in the code) and nearest neighbours (10 to 70 in steps of 5 in the code) chosen by maximal silhouette score, computed with the cosine metric in the code (the paper reports 5 clusters, 55 neighbours, silhouette 0.405). Proportions compared by chi-square test with Bonferroni correction over the five types (legend). Reproduced when the cluster with the latest response and longest decay holds no Vglut2 cells and its Vgat proportion differs at corrected p < 0.05, and the fast-response cluster holds a larger proportion of Vglut2 than of Vgat cells at corrected p < 0.05. The clustering has no fixed random seed in the code; cluster membership may vary between runs, which is reported.
- fig7fg-activation-suppresses
CNO activation of post-ejaculatory TRAP-labeled MPOA neurons reduces zone transitions and increases time in the isolation zone; activation of appetitive or consummatory ensembles and eYFP controls do not.
only post-ejaculatory-hM3Dq group showed significantly fewer number of transitions and spent more time in the isolation zone on the CNO trial than on the saline trial
Figure 7F and 7G; Results, An activity-defined subset of neurons in the MPOA, paragraph 4How it will be judged
Saline against CNO trial within each group, Bonferroni over the four groups. The legend names a Wilcoxon rank sum test; Figure 6.ipynb runs scipy wilcoxon (paired signed-rank), and 0.03125 is 4 times the smallest two-sided signed-rank p for 8 mice. Methods first; the rank-sum test is the main reading and the signed-rank test is the variant. Reproduced when only post-ejaculatory-hM3Dq is significant at corrected p < 0.05 in both measures.
- fig7kl-activation-intromission
Activation of post-ejaculatory TRAP neurons lowers intromissions and receptivity in the self-paced assay; the text also reports fewer self-grooming and mounting episodes, which the legend gives as p = 0.0625.
post-ejaculatory-hM3Dq group displayed fewer numbers of self-grooming, mounting, intromission and lower receptivity score on the CNO trial than on the saline trial
Figure 7H to 7L; Results, An activity-defined subset of neurons in the MPOA, paragraph 4How it will be judged
Tests as in fig7fg-activation-suppresses. Reproduced when intromission and receptive score are lower on CNO at corrected p < 0.05; grooming and mounting are reported.
- fig7s1-mpn-proportion
The number of TRAP-labeled cells does not differ between groups, but the proportion located in the MPN is higher in the post-ejaculatory group; most MPN TRAP cells are Vgat (0.76) and few Vglut2 (0.15).
while the overall number of TRAP-labeled cells had no difference between the post-ejaculatory groups, consummatory group and appetitive group, the proportion of cells located in the MPN was significantly higher in the post-ejaculatory group ... most TRAP-labeled cells in the MPN were Vgat (Vgat: 0.76±0.16. Vglut2: 0.15±0.13)
Figure 7-figure supplement 1B, 1C and 1G; Results, An activity-defined subset of neurons in the MPOA, paragraph 1How it will be judged
One-way ANOVA and Tukey HSD as in the legend (Figure S9.ipynb, pairwise_tukeyhsd). Tolerances are the stated spread. The text does not say over which mice the 0.76 and 0.15 are taken; the readings are (a) the appetitive-hM3Dq and post-ejaculatory-hM3Dq mice of panel G pooled (13), (b) the post-ejaculatory mice alone (7). The Vgat against Vglut2 test of G is not named; the degrees of freedom (2n - 2) fit an unpaired Student's t-test, which is the main reading, with the paired test as a variant. Reproduced when the count ANOVA is not significant, the MPN proportion ANOVA is, post-ejaculatory above appetitive at p < 0.05, the Vgat proportion exceeds the Vglut2 proportion at p < 0.05 in both groups, and the proportions match under one reading.
- fig7s2-home-cage-activation
In the home cage, activation of post-ejaculatory TRAP neurons reduces sniffing and intromission compared with post-ejaculatory eYFP controls, without changing mounting; it does not induce place preference or aversion.
post-ejaculatory-hM3Dq group but not the appetitive-hM3Dq and consummatory-hM3Dq group showed significantly fewer sniffing events compared to post-ejaculatory-eYFP group ... there were significantly less intromission episodes
Figure 7-figure supplement 2C to 2J; Results, An activity-defined subset of neurons in the MPOA, paragraphs 3 and 4How it will be judged
Each hM3Dq group against post-ejaculatory-eYFP by Mann-Whitney U test with Bonferroni correction (legend; Figure S10.ipynb). Reproduced when sniff and intromission are significant for post-ejaculatory-hM3Dq only, and mounting and the place preference measures are not.
- fig7s3-inhibition
Chemogenetic inhibition of post-ejaculatory TRAP neurons does not change behavior in the self-paced assay, but increases intromission and receptivity in the home cage assay.
the post-ejaculatory-hM4Di group experienced did not show significant changes in sniffing, self-grooming, or mounting, but showed more intromission and higher sexual receptivity than in the post-ejaculatory-eYFP group
Figure 7-figure supplement 3; Results, An activity-defined subset of neurons in the MPOA, paragraph 5How it will be judged
Home cage, post-ejaculatory-hM4Di against post-ejaculatory-eYFP by two-sided Mann-Whitney U test without correction (legend, O to R; Figure S11.ipynb, scipy mannwhitneyu). Self-paced assay, saline against CNO trial within each group with Bonferroni correction (E to L); the legend names a Wilcoxon rank sum test and Figure S11.ipynb runs scipy wilcoxon (paired signed-rank), so, as in fig7fg-activation-suppresses, the rank-sum test is the main reading and the signed-rank test the variant. The absence in the self-paced assay is judged on the main reading. Reproduced when both home cage measures are higher in hM4Di at p < 0.05, sniffing and mounting are not, and no self-paced measure is significant.
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